Overexpression of the prosystemin gene in transgenic tomato plants generates a systemic signal that constitutively induces proteinase inhibitor synthesis.
نویسندگان
چکیده
Tomato plants (Lycopersicon esculentum, var. Better Boy) were stably transformed with a gene consisting of the open reading frame of a prosystemin cDNA under the regulation of the cauliflower mosaic virus 35S promoter. The leaves of the transgenic plants constitutively produced proteinase inhibitor I and II proteins, which accumulated over time to levels exceeding 1 mg/g of dry leaf weight. This phenotype contrasts with that of untransformed plants, which produce proteinase inhibitor proteins in leaves only in response to wounding or chemical inducers. The transgenic plants were also stunted, although they appeared normal in all other respects. Grafting the upper half (scion) of an untransformed tomato plant onto the lower half (root stock) of a tomato plant expressing the prosystemin transgene resulted in the constitutive expression of proteinase inhibitor proteins in the leaves of both the transformed root stock and the untransformed scion, demonstrating that expression of the prosystemin transgene generates a mobile wound signal. These results show that systemic signal propagation in the transgenic plants does not require wounding, and they support the proposed role of systemin as the mobile wound signal.
منابع مشابه
Prosystemin overexpression induces transcriptional modifications of defense-related and receptor-like kinase genes and reduces the susceptibility to Cucumber mosaic virus and its satellite RNAs in transgenic tomato plants
Systemin is a plant signal peptide hormone involved in the responses to wounding and insect damage in the Solanaceae family. It works in the same signaling pathway of jasmonic acid (JA) and enhances the expression of proteinase inhibitors. With the aim of studying a role for systemin in plant antiviral responses, a tomato (Solanum lycopersicum) transgenic line overexpressing the prosystemin cDN...
متن کاملGenetic analysis of wound signaling in tomato. Evidence for a dual role of jasmonic acid in defense and female fertility.
Genetic analysis of the wound response pathway in tomato (Lycopersicon esculentum) indicates that prosystemin and systemin are upstream components of a defensive signaling cascade that involves complex regulation of jasmonic acid (JA) biosynthesis and the ability of cells to perceive and respond to JA. Recent identification of JA response mutants provides evidence for the hypothesis that the JA...
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In tomato plants, systemic induction of defense genes in response to herbivory or mechanical wounding is regulated by an 18-amino-acid peptide signal called systemin. Transgenic plants that overexpress prosystemin, the systemin precursor, from a 35S::prosystemin (35S::prosys) transgene exhibit constitutive expression of wound-inducible defense proteins including proteinase inhibitors and polyph...
متن کاملScientific Correspondence Genetic Analysis of Wound Signaling in Tomato. Evidence for a Dual Role of Jasmonic Acid in Defense and Female Fertility
Genetic analysis of the wound response pathway in tomato (Lycopersicon esculentum) indicates that prosystemin and systemin are upstream components of a defensive signaling cascade that involves complex regulation of jasmonic acid (JA) biosynthesis and the ability of cells to perceive and respond to JA. Recent identification of JA response mutants provides evidence for the hypothesis that the JA...
متن کاملSystemic wound signaling in plants: a new perception.
O 100 species of plants exhibit systemic wound signaling that induces the production of defensive chemicals in leaves and stems (1). The signaling is caused by herbivore attacks and also occurs in response to some pathogens (2–5). The most intensively studied systemic signaling response is that found in species of the Solanaceae family, where a systemic wound signal that is graft transmissible ...
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ورودعنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 91 21 شماره
صفحات -
تاریخ انتشار 1994